Safe Mycobacterium tuberculosis DNA Extraction Method That Does Not Compromise Integrity
نویسندگان
چکیده
منابع مشابه
Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity.
Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist.
متن کاملInactivation of cultured Mycobacterium tuberculosis organisms prior to DNA extraction.
Somerville and his colleagues have made an important point that current protocols for DNA extraction from cultured Mycobacterium tuberculosis organisms do not ensure complete inactivation of the microorganism (9). While diagnosis samples of tuberculosis can be manipulated under biosafety level 2 (BSL2) conditions, live cultured M. tuberculosis organisms have to be manipulated under BSL3 conditi...
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DNA fingerprinting of Mycobacterium tuberculosis by IS6110 restriction fragment length polymorphism analysis requires substantial high-quality DNA. We demonstrated that, despite extraction treatments that might be expected to inactivate this organism, M. tuberculosis remained viable during this process. These data suggest that the extraction of M. tuberculosis DNA should be performed within con...
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BACKGROUND Chronic helminth infections induce a Th2 immune shift and establish an immunoregulatory milieu. As both of these responses can suppress Th1 immunity, which is necessary for control of Mycobacterium tuberculosis (MTB) infection, we hypothesized that chronic helminth infections may exacerbate the course of MTB. METHODOLOGY/PRINCIPAL FINDINGS Co-infection studies were conducted in cot...
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Fluoroquinolones are gaining increasing importance in the treatment of tuberculosis. The expression of MfpA, a member of the pentapeptide repeat family of proteins from Mycobacterium tuberculosis, causes resistance to ciprofloxacin and sparfloxacin. This protein binds to DNA gyrase and inhibits its activity. Its three-dimensional structure reveals a fold, which we have named the right-handed qu...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2006
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.44.1.254-256.2006